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Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi; Furusawa, Yoshiya*; Aoki, Mizuho*; Shao, C.*; Yokota, Yuichiro; Sakashita, Tetsuya; Matsumoto, Yoshitaka*; Kakizaki, Takehiko; et al.
Uchu Seibutsu Kagaku, 18(4), p.235 - 240, 2004/12
no abstracts in English
Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi; Sakashita, Tetsuya
Uchu Seibutsu Kagaku, 18(3), p.186 - 187, 2004/11
no abstracts in English
Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi*; Taguchi, Mitsumasa; Watanabe, Hiroshi
Radiation Research, 161(1), p.90 - 91, 2004/01
A single cell irradiation system has been developed for targeting cells individually with a precise number of heavy ions to elucidate radiobiological effects of exactly one particle and to investigate the biological effects of low fluence irradiation with HZE particles. Using the heavy ion microbeam apparatus installed at JAERI-Takasaki, mammalian cells were irradiated in the atmosphere with a single or precise numbers of ions, 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar. The number of ions traversed the cells attached on the ion track detector CR-39 were counted with a plastic scintillator. Immediately after the irradiation, the position and the number of ion tracks traversed the cell was detected with etching of CR-39 from the opposite side of the cell with alkaline-ethanol solution at 37
C. The growths of the cells were observed individually up to 60 hours after irradiation.
Kobayashi, Yasuhiko; Funayama, Tomoo; Wada, Seiichi; Taguchi, Mitsumasa; Watanabe, Hiroshi
Nuclear Instruments and Methods in Physics Research B, 210(1-4), p.308 - 311, 2003/09
A method for detecting the ion hit tracks on the mammalian cultured cells at the irradiation time was established. The cells were attached to the ion track detector CR-39 (100 
m thick), then irradiated with 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar ion beams. Immediately after the irradiation, the cells were refilled with medium, then the CR-39 was etched from the opposite side of the cell with alkaline-ethanol solution at 37C. With the 15 min etching treatment, we obtained the accurate information about the spatial distribution of irradiated ions without significant effect on the cell growth. The continuous observation of the individual cell growth indicated that the growth of ion hit cell was reduced compared with that of non-irradiated one.
Kobayashi, Yasuhiko; Funayama, Tomoo
Isotope News, (590), p.2 - 7, 2003/06
A microbeam can be used for selective irradiation of individual cells, which can be subsequently observed to ascertain what changes occur to that cell and to neighboring un-irradiated cells. Therefore, the use of microbeam allows direct investigation of cell-to-cell communications such as "bystander effects", that is, radiation effects transmitted from irradiated cells to neighboring un-irradiated cells. Furthermore, a microbeam with sufficient spatial resolution will be useful for analyzing the dynamics of intra-cellular process such as apoptosis and the influence of track-structure of energetic heavy ions by means of highly localized irradiation of a part of a nucleus or cytoplasm.
Wada, Seiichi; Natsuhori, Masahiro*; Ito, Nobuhiko*; Funayama, Tomoo; Kobayashi, Yasuhiko
Nuclear Instruments and Methods in Physics Research B, 206, p.553 - 556, 2003/05
Times Cited Count：3 Percentile：27.66(Instruments & Instrumentation)Determining the biological effects of a very low number of charged particles crossing the cell nucleus is interest for estimating the risk due to environmental exposure to charged particles. Especially it is necessary to detect the radiation damage induced by a precise number of charged particles in the individual cells. To compare the number of ions traversing the cell and the DNA damage produced by the hit ions, we applied comet assay. Cells attached on the ion track detector CR-39 were irradiated with 17.3 MeV/u 12C, 15.7 MeV/u, 10.4 MeV/u 20Ne and 6.9 MeV/u 40Ar ion beams at TIARA, JAERI-Takasaki. After irradiation, CR-39 was covered with 1 % agarose. After electrophoresis the CR-39 was taken off from the slide glass. The agarose gel on the CR-39 was stained with ethidium bromide and the opposite side of the CR-39 was etched with KOH-ethanol solution at 37 
. We observed that the ion particles with higher LET value induced the heavier DNA damage, even by the same number of ion-hits within the irradiated cells.
Kobayashi, Yasuhiko
Hoshasen Seibutsu Kenkyu, 37(1), p.67 - 84, 2002/03
A single cell irradiation system has been developed for targeting cells individually with a precise number of high-LET heavy ions to elucidate radiobiological effects of exactly one particle and to investigate the interaction of damages produced by separate events. Using the heavy ion microbeam apparatus in TIARA, mammalian cells were irradiated in the atmosphere with a single or precise numbers of heavy ions, 13.0 MeV/u 20Ne or 11.5 MeV/u 40Ar, with a spatial resolution of a few microns.
Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Wada, Seiichi*; Funayama, Tomoo; Khoa, T. V.; Kamiya, Tomihiro; Watanabe, Hiroshi; Yamamoto, Kazuo
JAERI-Review 2000-024, TIARA Annual Report 1999, p.48 - 50, 2000/10
no abstracts in English
Watanabe, Hiroshi; Kobayashi, Yasuhiko
Isotope News, (549), p.2 - 5, 2000/02
no abstracts in English
Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi; Yamamoto, Kazuo
JAERI-Review 99-025, TIARA Annual Report 1998, p.50 - 52, 1999/10
no abstracts in English
